8PN9

Structure of human oligosaccharyltransferase OST-A complex bound to NGI-1

Summary for 8PN9

• Entry DOI: 10.2210/pdb8pn9/pdb
• EMDB information: 17779
• Descriptor: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (18 entities in total)
• Functional Keywords: n-glycosylation, ost-a complex, ngi-1 inhibitor, transferase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 321362.22

Authors

Ramirez, A.S.,Kowal, J.,Locher, K.P. (deposition date: 2023-06-30, release date: 2024-05-08)

• Primary citation: Lampson, B.L.,Ramirez, A.S.,Baro, M.,He, L.,Hegde, M.,Koduri, V.,Pfaff, J.L.,Hanna, R.E.,Kowal, J.,Shirole, N.H.,He, Y.,Doench, J.G.,Contessa, J.N.,Locher, K.P.,Kaelin Jr., W.G.; Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-kappa B.; Cell, 187:2209-2223.e16, 2024; PubMed: 38670073; DOI: 10.1016/j.cell.2024.03.022

Experimental method

ELECTRON MICROSCOPY (3.61 Å)