8PM2

Structure of the murine trace amine-associated receptor TAAR7f bound to N,N-dimethylcyclohexylamine (DMCH) in complex with mini-Gs trimeric G protein

8PM2 の概要

• エントリーDOI: 10.2210/pdb8pm2/pdb
• EMDBエントリー: 17756
• 分子名称: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (7 entities in total)
• 機能のキーワード: trace-amine associated receptor, taar, mtaar7f, gpcr, receptor, g protein, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 132705.17

構造登録者

Gusach, A.,Lee, Y.,Edwards, P.C.,Huang, F.,Weyand, S.N.,Tate, C.G. (登録日: 2023-06-28, 公開日: 2023-08-09, 最終更新日: 2025-07-09)

主引用文献

Gusach, A.,Lee, Y.,Khoshgrudi, A.N.,Mukhaleva, E.,Ma, N.,Koers, E.J.,Chen, Q.,Edwards, P.C.,Huang, F.,Kim, J.,Mancia, F.,Verprintsev, D.B.,Vaidehi, N.,Weyand, S.N.,Tate, C.G.
Molecular recognition of an aversive odorant by the murine trace amine-associated receptor TAAR7f.
Biorxiv, 2023

PubMed Abstract: There are two main families of G protein-coupled receptors that detect odours in humans, the odorant receptors (ORs) and the trace amine-associated receptors (TAARs). Their amino acid sequences are distinct, with the TAARs being most similar to the aminergic receptors such as those activated by adrenaline, serotonin and histamine. To elucidate the structural determinants of ligand recognition by TAARs, we have determined the cryo-EM structure of a murine receptor, mTAAR7f, coupled to the heterotrimeric G protein G and bound to the odorant N,N-dimethylcyclohexylamine (DMCH) to an overall resolution of 2.9 Å. DMCH is bound in a hydrophobic orthosteric binding site primarily through van der Waals interactions and a strong charge-charge interaction between the tertiary amine of the ligand and an aspartic acid residue. This site is distinct and non-overlapping with the binding site for the odorant propionate in the odorant receptor OR51E2. The structure, in combination with mutagenesis data and molecular dynamics simulations suggests that the activation of the receptor follows a similar pathway to that of the β-adrenoceptors, with the significant difference that DMCH interacts directly with one of the main activation microswitch residues.

PubMed: 37461561
DOI: 10.1101/2023.07.07.547762

実験手法

ELECTRON MICROSCOPY (2.92 Å)