8PK6

INTS13 complex with ZNF655

8PK6 の概要

• エントリーDOI: 10.2210/pdb8pk6/pdb
• 分子名称: Integrator complex subunit 13, Zinc finger protein 655 (2 entities in total)
• 機能のキーワード: protein complex, dna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 93647.47

構造登録者

Sabath, K.,Jonas, S. (登録日: 2023-06-25, 公開日: 2024-07-03, 最終更新日: 2024-11-13)

主引用文献

Sabath, K.,Nabih, A.,Arnold, C.,Moussa, R.,Domjan, D.,Zaugg, J.B.,Jonas, S.
Basis of gene-specific transcription regulation by the Integrator complex.
Mol.Cell, 84:2525-, 2024

PubMed Abstract: The Integrator complex attenuates gene expression via the premature termination of RNA polymerase II (RNAP2) at promoter-proximal pausing sites. It is required for stimulus response, cell differentiation, and neurodevelopment, but how gene-specific and adaptive regulation by Integrator is achieved remains unclear. Here, we identify two sites on human Integrator subunits 13/14 that serve as binding hubs for sequence-specific transcription factors (TFs) and other transcription effector complexes. When Integrator is attached to paused RNAP2, these hubs are positioned upstream of the transcription bubble, consistent with simultaneous TF-promoter tethering. The TFs co-localize with Integrator genome-wide, increase Integrator abundance on target genes, and co-regulate responsive transcriptional programs. For instance, sensory cilia formation induced by glucose starvation depends on Integrator-TF contacts. Our data suggest TF-mediated promoter recruitment of Integrator as a widespread mechanism for targeted transcription regulation.

PubMed: 38906142
DOI: 10.1016/j.molcel.2024.05.027

実験手法

X-RAY DIFFRACTION (3.21 Å)