8PFQ

RNA structure with 1-methylpseudouridine, P1 space group

Summary for 8PFQ

• Entry DOI: 10.2210/pdb8pfq/pdb
• Related: 8PFK
• Descriptor: Chains: A,B, MAGNESIUM ION (3 entities in total)
• Functional Keywords: sars-cov-2, covid-19, coronavirus, mrna vaccine, rna
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 7803.69

Authors

Spingler, B.,McAuley, K.,Nievergelt, P.,Thorn, A. (deposition date: 2023-06-16, release date: 2024-01-31, Last modification date: 2024-11-06)

Primary citation

Nievergelt, P.,Berliat, F.,McAuley, K.E.,Dorgan, C.R.,van Well, R.M.,Thorn, A.,Spingler, B.
RNA oligomers at atomic resolution containing 1-methylpseudouridine, an essential building block of mRNA vaccines.
Chemmedchem, 19:e202300600-e202300600, 2024

PubMed Abstract: All widely used mRNA vaccines against COVID-19 contain in their sequence 1-methylpseudouridine (m1Ψ) instead of uridine. In this publication, we report two high resolution crystal structures (at up to 1.01 and 1.32 Å, respectively) of one such double-stranded 12-mer RNA sequence crystallized in two crystal forms. The structures are compared with similar structures which do not contain this modification. Additionally, the X-ray structure of 1-methyl-pseudouridine itself was determined.

PubMed: 38235959
DOI: 10.1002/cmdc.202300600

Experimental method

X-RAY DIFFRACTION (1.01 Å)