8PFK
RNA structure with 1-methylpseudouridine, C2 space group
Summary for 8PFK
| Entry DOI | 10.2210/pdb8pfk/pdb |
| Descriptor | RNA (12-mer), MAGNESIUM ION (3 entities in total) |
| Functional Keywords | sars-cov-2, covid-19, coronavirus, mrna vaccine, rna |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 3914.00 |
| Authors | Spingler, B.,McAuley, K.,Nievergelt, P.,Thorn, A. (deposition date: 2023-06-16, release date: 2024-01-31, Last modification date: 2025-02-19) |
| Primary citation | Nievergelt, P.,Berliat, F.,McAuley, K.E.,Dorgan, C.R.,van Well, R.M.,Thorn, A.,Spingler, B. RNA oligomers at atomic resolution containing 1-methylpseudouridine, an essential building block of mRNA vaccines. Chemmedchem, 19:e202300600-e202300600, 2024 Cited by PubMed Abstract: All widely used mRNA vaccines against COVID-19 contain in their sequence 1-methylpseudouridine (m1Ψ) instead of uridine. In this publication, we report two high resolution crystal structures (at up to 1.01 and 1.32 Å, respectively) of one such double-stranded 12-mer RNA sequence crystallized in two crystal forms. The structures are compared with similar structures which do not contain this modification. Additionally, the X-ray structure of 1-methyl-pseudouridine itself was determined. PubMed: 38235959DOI: 10.1002/cmdc.202300600 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.321 Å) |
Structure validation
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