8PDZ

Recombinant Ena3A L-Type endospore appendages

8PDZ の概要

• エントリーDOI: 10.2210/pdb8pdz/pdb
• EMDBエントリー: 17627
• 分子名称: DUF3992 domain-containing protein (1 entity in total)
• 機能のキーワード: endospore, pili, protein, fiber, spore, appendage, bacillus paranthracis, protein fibril, recombinant
• 由来する生物種: Bacillus paranthracis
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 324440.20

構造登録者

Sleutel, M.,Remaut, H. (登録日: 2023-06-13, 公開日: 2023-11-08, 最終更新日: 2025-09-10)

主引用文献

Sleutel, M.,Zegeye, E.D.,Llarena, A.K.,Pradhan, B.,Fislage, M.,O'Sullivan, K.,Van Gerven, N.,Aspholm, M.,Remaut, H.
Helical ultrastructure of the L-ENA spore aggregation factor of a Bacillus paranthracis foodborne outbreak strain.
Nat Commun, 15:7514-7514, 2024

PubMed Abstract: In pathogenic Bacillota, spores can form an infectious particle and can take up a central role in the environmental persistence and dissemination of disease. A poorly understood aspect of spore-mediated infection is the fibrous structures or 'endospore appendages' (ENAs) that have been seen to decorate the spores of pathogenic Bacilli and Clostridia. Current methodological approaches are opening a window on these long enigmatic structures. Using cryoID, Alphafold modelling and genetic approaches we identify a sub-class of robust ENAs in a Bacillus paranthracis foodborne outbreak strain. We demonstrate that L-ENA are encoded by a rare three-gene cluster (ena3) that contains all components for the self-assembly of ladder-like protein nanofibers of stacked heptameric rings, their anchoring to the exosporium, and their termination in a trimeric 'ruffle' made of a complement C1Q-like BclA paralogue. The role of ENA fibers in spore-spore interaction and the distribution of L-ENA operon as mobile genetic elements in B. cereus s.l. strains suggest that L-ENA fibers may increase the survival, spread and virulence of these strains.

PubMed: 39209852
DOI: 10.1038/s41467-024-51804-w

実験手法

ELECTRON MICROSCOPY (3.32 Å)