Summary for 8P5M
| Entry DOI | 10.2210/pdb8p5m/pdb |
| EMDB information | 17451 |
| Descriptor | Spike protein S1, Mab-23 (Heavy chain variable domain), Mab-23 (Light chain variable domain) (3 entities in total) |
| Functional Keywords | antibody, spike, complex, viral protein |
| Biological source | Severe acute respiratory syndrome coronavirus 2 More |
| Total number of polymer chains | 3 |
| Total formula weight | 93737.93 |
| Authors | Das, H.,Hallberg, B.M. (deposition date: 2023-05-24, release date: 2024-06-05, Last modification date: 2024-12-18) |
| Primary citation | Mandolesi, M.,Das, H.,de Vries, L.,Yang, Y.,Kim, C.,Dhinakaran, M.,Castro Dopico, X.,Fischbach, J.,Kim, S.,Guryleva, M.V.,Adori, M.,Chernyshev, M.,Stalmarck, A.,Hanke, L.,McInerney, G.M.,Sheward, D.J.,Corcoran, M.,Hallberg, B.M.,Murrell, B.,Karlsson Hedestam, G.B. Multi-compartmental diversification of neutralizing antibody lineages dissected in SARS-CoV-2 spike-immunized macaques. Nat Commun, 15:6338-6338, 2024 Cited by PubMed Abstract: The continued evolution of SARS-CoV-2 underscores the need to understand qualitative aspects of the humoral immune response elicited by spike immunization. Here, we combine monoclonal antibody (mAb) isolation with deep B cell receptor (BCR) repertoire sequencing of rhesus macaques immunized with prefusion-stabilized spike glycoprotein. Longitudinal tracing of spike-sorted B cell lineages in multiple immune compartments demonstrates increasing somatic hypermutation and broad dissemination of vaccine-elicited B cells in draining and non-draining lymphoid compartments, including the bone marrow, spleen and, most notably, periaortic lymph nodes. Phylogenetic analysis of spike-specific monoclonal antibody lineages identified through deep repertoire sequencing delineates extensive intra-clonal diversification that shaped neutralizing activity. Structural analysis of the spike in complex with a broadly neutralizing mAb provides a molecular basis for the observed differences in neutralization breadth between clonally related antibodies. Our findings highlight that immunization leads to extensive intra-clonal B cell evolution where members of the same lineage can both retain the original epitope specificity and evolve to recognize additional spike variants not previously encountered. PubMed: 39068149DOI: 10.1038/s41467-024-50286-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.8 Å) |
Structure validation
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