8P45

Crystal structure of human STING in complex with the agonist MD1202D

8P45 の概要

• エントリーDOI: 10.2210/pdb8p45/pdb
• 分子名称: Stimulator of interferon genes protein, 9-[(1~{S},3~{R},6~{R},8~{R},9~{R},10~{R},12~{R},15~{R},17~{R},18~{R})-8-(6-aminopurin-9-yl)-9,18-bis(fluoranyl)-3,12-bis(oxidanylidene)-3,12-bis(sulfanyl)-2,4,7,11,13-pentaoxa-3$l^{5},12$l^{5}-diphosphatricyclo[13.2.1.0^{6,10}]octadecan-17-yl]-1~{H}-purin-6-one (2 entities in total)
• 機能のキーワード: sting, antiviral, activator, antiviral protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23882.60

構造登録者

Klima, M.,Boura, E. (登録日: 2023-05-19, 公開日: 2023-12-20, 最終更新日: 2024-04-17)

主引用文献

Klima, M.,Dejmek, M.,Duchoslav, V.,Eisenreichova, A.,Sala, M.,Chalupsky, K.,Chalupska, D.,Novotna, B.,Birkus, G.,Nencka, R.,Boura, E.
Fluorinated cGAMP analogs, which act as STING agonists and are not cleavable by poxins: Structural basis of their function.
Structure, 32:433-, 2024

PubMed Abstract: The cGAS-STING pathway is a crucial part of innate immunity; it serves to detect DNA in the cytoplasm and to defend against certain cancers, viruses, and bacteria. We designed and synthesized fluorinated carbocyclic cGAMP analogs, MD1203 and MD1202D (MDs), to enhance their stability and their affinity for STING. These compounds demonstrated exceptional activity against STING. Despite their distinct chemical modifications relative to the canonical cyclic dinucleotides (CDNs), crystallographic analysis revealed a binding mode with STING that was consistent with the canonical CDNs. Importantly, MDs were resistant to cleavage by viral poxin nucleases and MDs-bound poxin adopted an unliganded-like conformation. Moreover, MDs complexed with poxin showed a conformation distinct from cGAMP bound to poxin, closely resembling their conformation when bound to STING. In conclusion, the development of MD1203 and MD1202D showcases their potential as potent STING activators with remarkable stability against poxin-mediated degradation-a crucial characteristic for future development of antivirals.

PubMed: 38325369
DOI: 10.1016/j.str.2024.01.008

実験手法

X-RAY DIFFRACTION (3.23 Å)