8P2E
Homotypic interacting B1 fab bound to Chondroitin Sulfate A
Summary for 8P2E
Entry DOI | 10.2210/pdb8p2e/pdb |
EMDB information | 17362 |
Descriptor | B1 fab light, B1 fab heavy (2 entities in total) |
Functional Keywords | chondroitin sulfate a, sugar binding protein |
Biological source | Homo sapiens More |
Total number of polymer chains | 8 |
Total formula weight | 200327.50 |
Authors | Raghavan, S.S.R.,Dagil, R.,Wang, K.T.,Salanti, A. (deposition date: 2023-05-16, release date: 2024-06-05, Last modification date: 2025-07-02) |
Primary citation | Vidal-Calvo, E.E.,Martin-Salazar, A.,Choudhary, S.,Dagil, R.,Raghavan, S.S.R.,Duvnjak, L.,Nordmaj, M.A.,Clausen, T.M.,Skafte, A.,Oberkofler, J.,Wang, K.,Agerbaek, M.O.,Loppke, C.,Jorgensen, A.M.,Ropac, D.,Mujollari, J.,Willis, S.,Garcias Lopez, A.,Miller, R.L.,Karlsson, R.T.G.,Goerdeler, F.,Chen, Y.H.,Colaco, A.R.,Wang, Y.,Lavstsen, T.,Martowicz, A.,Nelepcu, I.,Marzban, M.,Oo, H.Z.,Orum-Madsen, M.S.,Wang, Y.,Nielsen, M.A.,Clausen, H.,Wierer, M.,Wolf, D.,Gogenur, I.,Theander, T.G.,Al-Nakouzi, N.,Gustavsson, T.,Daugaard, M.,Salanti, A. Tumor-agnostic cancer therapy using antibodies targeting oncofetal chondroitin sulfate. Nat Commun, 15:7553-7553, 2024 Cited by PubMed Abstract: Molecular similarities between embryonic and malignant cells can be exploited to target tumors through specific signatures absent in healthy adult tissues. One such embryonic signature tumors express is oncofetal chondroitin sulfate (ofCS), which supports disease progression and dissemination in cancer. Here, we report the identification and characterization of phage display-derived antibody fragments recognizing two distinct ofCS epitopes. These antibody fragments show binding affinity to ofCS in the low nanomolar range across a broad selection of solid tumor types in vitro and in vivo with minimal binding to normal, inflamed, or benign tumor tissues. Anti-ofCS antibody drug conjugates and bispecific immune cell engagers based on these targeting moieties disrupt tumor progression in animal models of human and murine cancers. Thus, anti-ofCS antibody fragments hold promise for the development of broadly effective therapeutic and diagnostic applications targeting human malignancies. PubMed: 39215044DOI: 10.1038/s41467-024-51781-0 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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