8P1B

Lysozyme structure solved from serial crystallography data collected at 2 kHz with JUNGFRAU detector at MAXIV

Summary for 8P1B

• Entry DOI: 10.2210/pdb8p1b/pdb
• Related: 8P1A 8P1C 8P1D
• Descriptor: Lysozyme C, 1,2-ETHANEDIOL, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: serial crystallography, jungfrau detector, 2khz frame rate, hydrolase
• Biological source: Gallus gallus (chicken)
• Total number of polymer chains: 1
• Total formula weight: 14526.20

Authors

Nan, J.,Leonarski, F.,Furrer, A.,Dworkowski, F. (deposition date: 2023-05-11, release date: 2023-10-18, Last modification date: 2024-11-06)

Primary citation

Leonarski, F.,Nan, J.,Matej, Z.,Bertrand, Q.,Furrer, A.,Gorgisyan, I.,Bjelcic, M.,Kepa, M.,Glover, H.,Hinger, V.,Eriksson, T.,Cehovin, A.,Eguiraun, M.,Gasparotto, P.,Mozzanica, A.,Weinert, T.,Gonzalez, A.,Standfuss, J.,Wang, M.,Ursby, T.,Dworkowski, F.
Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source.
Iucrj, 10:729-737, 2023

PubMed Abstract: Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourth-generation synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing.

PubMed: 37830774
DOI: 10.1107/S2052252523008618

Experimental method

X-RAY DIFFRACTION (1.7 Å)