8OZM

In situ cryoEM structure of the Prototype Foamy Virus capsid, hexamer 1 localised reconstruction

8OZM の概要

• エントリーDOI: 10.2210/pdb8ozm/pdb
• 関連するPDBエントリー: 8OZH 8OZJ 8OZK 8OZL 8OZM 8OZN 8OZP 8OZQ
• EMDBエントリー: 17309 17311 17312 17313 17314 17315 17316 17317 17318 17319 17320 17321 17322
• 分子名称: Gag polyprotein (1 entity in total)
• 機能のキーワード: capsid, gag, foamy virus, hexamer, viral protein
• 由来する生物種: Eastern chimpanzee simian foamy virus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 424160.48

構造登録者

Calcraft, T.,Nans, A.,Rosenthal, P.B. (登録日: 2023-05-09, 公開日: 2024-07-10, 最終更新日: 2024-08-21)

主引用文献

Calcraft, T.,Stanke-Scheffler, N.,Nans, A.,Lindemann, D.,Taylor, I.A.,Rosenthal, P.B.
Integrated cryoEM structure of a spumaretrovirus reveals cross-kingdom evolutionary relationships and the molecular basis for assembly and virus entry.
Cell, 187:4213-4230.e19, 2024

PubMed Abstract: Foamy viruses (FVs) are an ancient lineage of retroviruses, with an evolutionary history spanning over 450 million years. Vector systems based on Prototype Foamy Virus (PFV) are promising candidates for gene and oncolytic therapies. Structural studies of PFV contribute to the understanding of the mechanisms of FV replication, cell entry and infection, and retroviral evolution. Here we combine cryoEM and cryoET to determine high-resolution in situ structures of the PFV icosahedral capsid (CA) and envelope glycoprotein (Env), including its type III transmembrane anchor and membrane-proximal external region (MPER), and show how they are organized in an integrated structure of assembled PFV particles. The atomic models reveal an ancient retroviral capsid architecture and an unexpected relationship between Env and other class 1 fusion proteins of the Mononegavirales. Our results represent the de novo structure determination of an assembled retrovirus particle.

PubMed: 39013471
DOI: 10.1016/j.cell.2024.06.017

実験手法

ELECTRON MICROSCOPY (3.4 Å)