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8OXV

Transglutaminase 3 zymogen in complex with DH patient-derived Fab DH63-B02

Summary for 8OXV
Entry DOI10.2210/pdb8oxv/pdb
DescriptorProtein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain, Antibody Fab fragment Heavy chain, Antibody Fab fragment light chain, ... (7 entities in total)
Functional Keywordstransglutaminase, tgm3, tg3, transglutaminase 3, enzyme, zymogen, antibody, dermatitis herpetiformis, transferase
Biological sourceHomo sapiens (human)
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Total number of polymer chains3
Total formula weight124310.81
Authors
Heggelund, J.E.,Sollid, L.M. (deposition date: 2023-05-02, release date: 2023-10-18, Last modification date: 2024-11-13)
Primary citationHeggelund, J.E.,Das, S.,Stamnaes, J.,Iversen, R.,Sollid, L.M.
Autoantibody binding and unique enzyme-substrate intermediate conformation of human transglutaminase 3.
Nat Commun, 14:6216-6216, 2023
Cited by
PubMed Abstract: Transglutaminase 3 (TG3), the autoantigen of dermatitis herpetiformis (DH), is a calcium dependent enzyme that targets glutamine residues in polypeptides for either transamidation or deamidation modifications. To become catalytically active TG3 requires proteolytic cleavage between the core domain and two C-terminal β-barrels (C1C2). Here, we report four X-ray crystal structures representing inactive and active conformations of human TG3 in complex with a TG3-specific Fab fragment of a DH patient derived antibody. We demonstrate that cleaved TG3, upon binding of a substrate-mimicking inhibitor, undergoes a large conformational change as a β-sheet in the catalytic core domain moves and C1C2 detaches. The unique enzyme-substrate conformation of TG3 without C1C2 is recognized by DH autoantibodies. The findings support a model where B-cell receptors of TG3-specific B cells bind and internalize TG3-gluten enzyme-substrate complexes thereby facilitating gluten-antigen presentation, T-cell help and autoantibody production.
PubMed: 37798283
DOI: 10.1038/s41467-023-42004-z
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.8 Å)
Structure validation

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