8OVA

CRYO-EM STRUCTURE OF TRYPANOSOMA BRUCEI PROCYCLIC FORM 80S RIBOSOME : PARENTAL STRAIN

This is a non-PDB format compatible entry.

Summary for 8OVA

• Entry DOI: 10.2210/pdb8ova/pdb
• EMDB information: 17208 17249 17250
• Descriptor: LSUa rRNA, E-SITE TRNA, Ribosomal protein S10, ... (91 entities in total)
• Functional Keywords: cryo-em, trypanosoma brucei, 80s ribosome, ribosome
• Biological source: Trypanosoma brucei brucei; More
• Total number of polymer chains: 86
• Total formula weight: 3661530.75

Authors

Rajan, K.S.,Yonath, A. (deposition date: 2023-04-25, release date: 2023-11-29, Last modification date: 2025-10-01)

Primary citation

Rajan, K.S.,Madmoni, H.,Bashan, A.,Taoka, M.,Aryal, S.,Nobe, Y.,Doniger, T.,Galili Kostin, B.,Blumberg, A.,Cohen-Chalamish, S.,Schwartz, S.,Rivalta, A.,Zimmerman, E.,Unger, R.,Isobe, T.,Yonath, A.,Michaeli, S.
A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.
Nat Commun, 14:7462-7462, 2023

PubMed Abstract: Trypanosomes are protozoan parasites that cycle between insect and mammalian hosts and are the causative agent of sleeping sickness. Here, we describe the changes of pseudouridine (Ψ) modification on rRNA in the two life stages of the parasite using four different genome-wide approaches. CRISPR-Cas9 knock-outs of all four snoRNAs guiding Ψ on helix 69 (H69) of the large rRNA subunit were lethal. A single knock-out of a snoRNA guiding Ψ530 on H69 altered the composition of the 80S monosome. These changes specifically affected the translation of only a subset of proteins. This study correlates a single site Ψ modification with changes in ribosomal protein stoichiometry, supported by a high-resolution cryo-EM structure. We propose that alteration in rRNA modifications could generate ribosomes preferentially translating state-beneficial proteins.

PubMed: 37985661
DOI: 10.1038/s41467-023-43263-6

Experimental method

ELECTRON MICROSCOPY (2.47 Å)