8OSI

Genetically encoded green ratiometric calcium indicator FNCaMP in calcium-bound state

Summary for 8OSI

• Entry DOI: 10.2210/pdb8osi/pdb
• Descriptor: mNeonGreen,Calmodulin,Contig An16c0100, genomic contig, CALCIUM ION (3 entities in total)
• Functional Keywords: fluorescent indicator, fluorescent sensor, chromophore, mneongreen, fluorescent protein
• Biological source: Branchiostoma lanceolatum; More
• Total number of polymer chains: 1
• Total formula weight: 52420.45

Authors

Varfolomeeva, L.A.,Boyko, K.M.,Nikolaeva, A.Y.,Subach, O.M.,Subach, F.V. (deposition date: 2023-04-19, release date: 2023-05-24, Last modification date: 2024-10-23)

Primary citation

Subach, O.M.,Varfolomeeva, L.,Vlaskina, A.V.,Agapova, Y.K.,Nikolaeva, A.Y.,Piatkevich, K.D.,Patrushev, M.V.,Boyko, K.M.,Subach, F.V.
FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.
Biochem.Biophys.Res.Commun., 665:169-177, 2023

PubMed Abstract: Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (LSS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator.

PubMed: 37163937
DOI: 10.1016/j.bbrc.2023.04.108

Experimental method

X-RAY DIFFRACTION (2.42 Å)