8ORG

Cryo-EM structure of SH-SY5Y seeded with filaments from corticobasal degeneration extracts (Type II)

Summary for 8ORG

• Entry DOI: 10.2210/pdb8org/pdb
• EMDB information: 17123
• Descriptor: Microtubule-associated protein tau (1 entity in total)
• Functional Keywords: amyloid, tau, protein fibril
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 3
• Total formula weight: 129104.36

Authors

Lovestam, S.,Scheres, S.H.W.,Goedert, M. (deposition date: 2023-04-13, release date: 2023-04-26, Last modification date: 2024-07-24)

Primary citation

Tarutani, A.,Lovestam, S.,Zhang, X.,Kotecha, A.,Robinson, A.C.,Mann, D.M.A.,Saito, Y.,Murayama, S.,Tomita, T.,Goedert, M.,Scheres, S.H.W.,Hasegawa, M.
Cryo-EM structures of tau filaments from SH-SY5Y cells seeded with brain extracts from cases of Alzheimer's disease and corticobasal degeneration.
Febs Open Bio, 13:1394-1404, 2023

PubMed Abstract: The formation of amyloid filaments through templated seeding is believed to underlie the propagation of pathology in most human neurodegenerative diseases. A widely used model system to study this process is to seed amyloid filament formation in cultured cells using human brain extracts. Here, we report the electron cryo-microscopy structures of tau filaments from  undifferentiated seeded SH-SY5Y cells that transiently expressed N-terminally HA-tagged 1N3R or 1N4R human tau, using brain extracts from individuals with Alzheimer's disease or corticobasal degeneration. Although the resulting filament structures differed from those of the brain seeds, some degrees of structural templating were observed. Studying templated seeding in cultured cells, and determining the structures of the resulting filaments, can thus provide insights into the cellular aspects underlying neurodegenerative diseases.

PubMed: 37337995
DOI: 10.1002/2211-5463.13657

Experimental method

ELECTRON MICROSCOPY (2.3 Å)