8OPP

Structure of human terminal uridylyltransferase 7 (hTUT7/ZCCHC6) bound with pre-let7g miRNA and UTPalphaS

8OPP の概要

• エントリーDOI: 10.2210/pdb8opp/pdb
• EMDBエントリー: 17084
• 分子名称: Terminal uridylyltransferase 7, RNA (25-MER), [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] phosphono hydrogen phosphate (3 entities in total)
• 機能のキーワード: polymerase, uridylation, rna maturation and turnover control, rna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 180001.74

構造登録者

Yi, G.,Ye, M.,Gilbert, R.J. (登録日: 2023-04-07, 公開日: 2024-07-24, 最終更新日: 2024-09-25)

主引用文献

Yi, G.,Ye, M.,Carrique, L.,El-Sagheer, A.,Brown, T.,Norbury, C.J.,Zhang, P.,Gilbert, R.J.C.
Structural basis for activity switching in polymerases determining the fate of let-7 pre-miRNAs.
Nat.Struct.Mol.Biol., 31:1426-1438, 2024

PubMed Abstract: Tumor-suppressor let-7 pre-microRNAs (miRNAs) are regulated by terminal uridylyltransferases TUT7 and TUT4 that either promote let-7 maturation by adding a single uridine nucleotide to the pre-miRNA 3' end or mark them for degradation by the addition of multiple uridines. Oligo-uridylation is increased in cells by enhanced TUT7/4 expression and especially by the RNA-binding pluripotency factor LIN28A. Using cryogenic electron microscopy, we captured high-resolution structures of active forms of TUT7 alone, of TUT7 plus pre-miRNA and of both TUT7 and TUT4 bound with pre-miRNA and LIN28A. Our structures reveal that pre-miRNAs engage the enzymes in fundamentally different ways depending on the presence of LIN28A, which clamps them onto the TUTs to enable processive 3' oligo-uridylation. This study reveals the molecular basis for mono- versus oligo-uridylation by TUT7/4, as determined by the presence of LIN28A, and thus their mechanism of action in the regulation of cell fate and in cancer.

PubMed: 39054354
DOI: 10.1038/s41594-024-01357-9

実験手法

ELECTRON MICROSCOPY (3.76 Å)