8ODV

Chaetomium thermophilum Get1/Get2 heterotetramer in complex with a Get3 dimer (nanodisc)

Summary for 8ODV

• Entry DOI: 10.2210/pdb8odv/pdb
• EMDB information: 16819
• Descriptor: ATPase GET3, Protein GET2,Protein GET1, ZINC ION (3 entities in total)
• Functional Keywords: membrane protein insertion, get pathway, tail anchored membrane protein, membrane protein
• Biological source: Thermochaetoides thermophila DSM 1495; More
• Total number of polymer chains: 4
• Total formula weight: 165467.65

Authors

McDowell, M.A.,Wild, K.,Sinning, I. (deposition date: 2023-03-09, release date: 2023-11-29)

Primary citation

McDowell, M.A.,Heimes, M.,Enkavi, G.,Farkas, A.,Saar, D.,Wild, K.,Schwappach, B.,Vattulainen, I.,Sinning, I.
The GET insertase exhibits conformational plasticity and induces membrane thinning.
Nat Commun, 14:7355-7355, 2023

PubMed Abstract: The eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum. In the cytosol, the Get3 chaperone captures the TA protein substrate and delivers it to the Get1/Get2 membrane protein complex (GET insertase), which then inserts the substrate via a membrane-embedded hydrophilic groove. Here, we present structures, atomistic simulations and functional data of human and Chaetomium thermophilum Get1/Get2/Get3. The core fold of the GET insertase is conserved throughout eukaryotes, whilst thinning of the lipid bilayer occurs in the vicinity of the hydrophilic groove to presumably lower the energetic barrier of membrane insertion. We show that the gating interaction between Get2 helix α3' and Get3 drives conformational changes in both Get3 and the Get1/Get2 membrane heterotetramer. Thus, we provide a framework to understand the conformational plasticity of the GET insertase and how it remodels its membrane environment to promote substrate insertion.

PubMed: 37963916
DOI: 10.1038/s41467-023-42867-2

Experimental method

ELECTRON MICROSCOPY (4.7 Å)