8KHF

Structure of the human ATP synthase bound to bedaquiline (membrane domain)

8KHF の概要

• エントリーDOI: 10.2210/pdb8khf/pdb
• EMDBエントリー: 37243
• 分子名称: ATP synthase F(0) complex subunit C1, mitochondrial, ATP synthase subunit f, mitochondrial, ATP synthase subunit g, mitochondrial, ... (13 entities in total)
• 機能のキーワード: atp synthase, human, cryo-em, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 225081.97

構造登録者

Lai, Y.,Zhang, Y. (登録日: 2023-08-21, 公開日: 2024-05-01, 最終更新日: 2024-08-21)

主引用文献

Zhang, Y.,Lai, Y.,Zhou, S.,Ran, T.,Zhang, Y.,Zhao, Z.,Feng, Z.,Yu, L.,Xu, J.,Shi, K.,Wang, J.,Pang, Y.,Li, L.,Chen, H.,Guddat, L.W.,Gao, Y.,Liu, F.,Rao, Z.,Gong, H.
Inhibition of M. tuberculosis and human ATP synthase by BDQ and TBAJ-587.
Nature, 631:409-414, 2024

PubMed Abstract: Bedaquiline (BDQ), a first-in-class diarylquinoline anti-tuberculosis drug, and its analogue, TBAJ-587, prevent the growth and proliferation of Mycobacterium tuberculosis by inhibiting ATP synthase. However, BDQ also inhibits human ATP synthase. At present, how these compounds interact with either M. tuberculosis ATP synthase or human ATP synthase is unclear. Here we present cryogenic electron microscopy structures of M. tuberculosis ATP synthase with and without BDQ and TBAJ-587 bound, and human ATP synthase bound to BDQ. The two inhibitors interact with subunit a and the c-ring at the leading site, c-only sites and lagging site in M. tuberculosis ATP synthase, showing that BDQ and TBAJ-587 have similar modes of action. The quinolinyl and dimethylamino units of the compounds make extensive contacts with the protein. The structure of human ATP synthase in complex with BDQ reveals that the BDQ-binding site is similar to that observed for the leading site in M. tuberculosis ATP synthase, and that the quinolinyl unit also interacts extensively with the human enzyme. This study will improve researchers' understanding of the similarities and differences between human ATP synthase and M. tuberculosis ATP synthase in terms of the mode of BDQ binding, and will allow the rational design of novel diarylquinolines as anti-tuberculosis drugs.

PubMed: 38961288
DOI: 10.1038/s41586-024-07605-8

実験手法

ELECTRON MICROSCOPY (3.13 Å)