8KFO

Crystal structure of BSA in complex with B3

Summary for 8KFO

• Entry DOI: 10.2210/pdb8kfo/pdb
• Descriptor: Albumin, 6-[(~{E})-2-[1-[2-[2-(2-methoxyethoxy)ethoxy]ethyl]pyridin-1-ium-4-yl]ethenyl]-~{N},~{N}-dimethyl-naphthalen-2-amine (2 entities in total)
• Functional Keywords: albumin dimer, photo-sensitizer, transport protein
• Biological source: Bos taurus (cattle)
• Total number of polymer chains: 2
• Total formula weight: 140900.56

Authors

Chen, X.,Ge, Y.H.,Yang, H.,Fang, B.,Li, L. (deposition date: 2023-08-16, release date: 2024-08-21, Last modification date: 2025-03-05)

Primary citation

Fang, B.,Bai, H.,Zhang, J.,Wang, L.,Li, P.,Ge, Y.,Yang, H.,Wang, H.,Peng, B.,Hu, W.,Ma, H.,Chen, X.,Fu, L.,Li, L.
Albumins constrainting the conformation of mitochondria-targeted photosensitizers for tumor-specific photodynamic therapy.
Biomaterials, 315:122914-122914, 2025

PubMed Abstract: Tumor ablation Preclinical organelle-targeted phototherapies have effectively achieved tumor photoablation for regenerative biomedical applications in cancer therapies. However, engineering effective phototherapy drugs with precise tumor-localization targeting and organelle direction remains challenging. Herein, we report a albumins constrainting mitochondrial-targeted photosensitizer nanoparticles (PSs@BSAs) for tumor-specific photodynamic therapy. X-ray crystallography elucidates the two-stage assembly mechanism of PSs@BSAs. Femtosecond transient absorption spectroscopy and quantum mechanical calculations reveal the implications of conformational dynamics at the excited state. PSs@BSAs can efficiently disable mitochondrial activity, and further disrupt tumor angiogenesis based on the photodynamic effect. This triggers a metabolic and oxidative stress crisis to facilitate photoablation of solid tumor and antitumor metastasis. The study fully elucidates the interdisciplinary issues of chemistry, physics, and biological interfaces, thereby opening new horizons to inspire the engineering of organelle-targeted tumor-specific photosensitizers for biomedical applications.

PubMed: 39461059
DOI: 10.1016/j.biomaterials.2024.122914

Experimental method

X-RAY DIFFRACTION (3.6 Å)