8KAM

Crystal structure of SpyCas9 in complex with sgRNA and 16nt target DNA

Summary for 8KAM

• Entry DOI: 10.2210/pdb8kam/pdb
• Descriptor: RNA (98-MER), CRISPR-associated endonuclease Cas9/Csn1, DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*CP*TP*TP*CP*AP*AP*TP*TP*AP*GP*AP*AP*CP*AP*CP*G)-3'), ... (5 entities in total)
• Functional Keywords: nuclease, rna binding protein/rna/dna, rna binding protein-rna-dna complex
• Biological source: Streptococcus pyogenes serotype M1; More
• Total number of polymer chains: 4
• Total formula weight: 200875.50

Authors

Chen, Y.,Chen, J.,Liu, L. (deposition date: 2023-08-03, release date: 2024-06-05, Last modification date: 2024-06-12)

• Primary citation: Chen, J.,Chen, Y.,Huang, L.,Lin, X.,Chen, H.,Xiang, W.,Liu, L.; Trans-nuclease activity of Cas9 activated by DNA or RNA target binding.; Nat.Biotechnol., 2024; PubMed: 38811761; DOI: 10.1038/s41587-024-02255-7

Experimental method

X-RAY DIFFRACTION (3.91 Å)