8KAG

Crystal structure of SpyCas9 in complex with sgRNA and target RNA

Summary for 8KAG

• Entry DOI: 10.2210/pdb8kag/pdb
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, RNA (98-MER), RNA (5'-R(*CP*CP*AP*CP*UP*UP*CP*AP*AP*UP*UP*AP*GP*AP*AP*CP*AP*CP*GP*GP*AP*CP*C)-3') (3 entities in total)
• Functional Keywords: nuclease, rna binding protein/rna, rna binding protein-rna complex
• Biological source: Streptococcus pyogenes serotype M1; More
• Total number of polymer chains: 3
• Total formula weight: 197489.94

Authors

Chen, Y.,Chen, J.,Liu, L. (deposition date: 2023-08-03, release date: 2024-06-05, Last modification date: 2024-06-12)

• Primary citation: Chen, J.,Chen, Y.,Huang, L.,Lin, X.,Chen, H.,Xiang, W.,Liu, L.; Trans-nuclease activity of Cas9 activated by DNA or RNA target binding.; Nat.Biotechnol., 2024; PubMed: 38811761; DOI: 10.1038/s41587-024-02255-7

Experimental method

X-RAY DIFFRACTION (3.88 Å)