8K9G

Cryo-EM structure of Crt-SPARTA-gRNA-tDNA dimer (conformation-1)

Summary for 8K9G

• Entry DOI: 10.2210/pdb8k9g/pdb
• EMDB information: 36986
• Descriptor: DNA (45-mer), RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*U)-3'), Piwi domain-containing protein, ... (4 entities in total)
• Functional Keywords: ago, dna/rna, dimer, dna binding protein, cell invasion, dna-rna-cell invasion complex, dna/rna/cell invasion
• Biological source: Thermoflavifilum thermophilum; More
• Total number of polymer chains: 8
• Total formula weight: 264190.99

Authors

Gao, X.,Shang, K.,Zhu, K.,Wang, L.,Mu, Z.,Fu, X.,Yu, X.,Qin, B.,Zhu, H.,Ding, W.,Cui, S. (deposition date: 2023-08-01, release date: 2023-10-18, Last modification date: 2024-02-07)

• Primary citation: Gao, X.,Shang, K.,Zhu, K.,Wang, L.,Mu, Z.,Fu, X.,Yu, X.,Qin, B.,Zhu, H.,Ding, W.,Cui, S.; Nucleic-acid-triggered NADase activation of a short prokaryotic Argonaute.; Nature, 625:822-831, 2024; PubMed: 37783228; DOI: 10.1038/s41586-023-06665-6

Experimental method

ELECTRON MICROSCOPY (3.49 Å)