8K4I
Crystal structure of YajQ STM0435
Summary for 8K4I
| Entry DOI | 10.2210/pdb8k4i/pdb |
| Descriptor | YajQ (2 entities in total) |
| Functional Keywords | structural genomics |
| Biological source | Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S |
| Total number of polymer chains | 1 |
| Total formula weight | 18344.84 |
| Authors | |
| Primary citation | Dai , Y.,Liu , R.,Yue , Y.,Song , N.,Jia , H.,Ma , Z.,Gao , X.,Zhang , M.,Yuan , X.,Liu , Q.,Liu , X.,Li , B.,Wang , W. A c-di-GMP binding effector STM0435 modulates flagellar motility and pathogenicity in Salmonella Virulence, 15:2331265-, 2024 Cited by PubMed Abstract: Flagella play a crucial role in the invasion process of and function as a significant antigen that triggers host pyroptosis. Regulation of flagellar biogenesis is essential for both pathogenicity and immune escape of . We identified the conserved and unknown function protein STM0435 as a new flagellar regulator. The ∆ strain exhibited higher pathogenicity in both cellular and animal infection experiments than the wild-type . Proteomic and transcriptomic analyses demonstrated dramatic increases in almost all flagellar genes in the ∆ strain compared to wild-type . In a surface plasmon resonance assay, purified STM0435 protein-bound c-di-GMP had an affinity of ~8.383 µM. The crystal structures of apo-STM0435 and STM0435&c-di-GMP complex were determined. Structural analysis revealed that R33, R137, and D138 of STM0435 were essential for c-di-GMP binding. A with STM1987 (GGDEF protein) or STM4264 (EAL protein) overexpression exhibits completely different motility behaviours, indicating that the binding of c-di-GMP to STM0435 promotes its inhibitory effect on flagellar biogenesis. PubMed: 38532247DOI: 10.1080/21505594.2024.2331265 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.48 Å) |
Structure validation
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