8K0K

Crystal structure of Csy complex

Summary for 8K0K

• Entry DOI: 10.2210/pdb8k0k/pdb
• Descriptor: Csy1, Csy2, Csy3, ... (5 entities in total)
• Functional Keywords: complex, rna-protein complex, rna binding protein/rna, rna binding protein-rna complex
• Biological source: Vibrio phage ICP1_2011_A; More
• Total number of polymer chains: 10
• Total formula weight: 285990.18

Authors

Feng, Y.,Wang, H. (deposition date: 2023-07-09, release date: 2024-07-10, Last modification date: 2025-01-22)

Primary citation

Zhang, L.,Wang, H.,Zeng, J.,Cao, X.,Gao, Z.,Liu, Z.,Li, F.,Wang, J.,Zhang, Y.,Yang, M.,Feng, Y.
Cas1 mediates the interference stage in a phage-encoded CRISPR-Cas system.
Nat.Chem.Biol., 20:1471-1481, 2024

PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems are prokaryotic adaptive immune systems against invading phages and other mobile genetic elements. Notably, some phages, including the Vibrio cholerae-infecting ICP1 (International Center for Diarrheal Disease Research, Bangladesh cholera phage 1), harbor CRISPR-Cas systems to counteract host defenses. Nevertheless, ICP1 Cas8f lacks the helical bundle domain essential for recruitment of helicase-nuclease Cas2/3 during target DNA cleavage and how this system accomplishes the interference stage remains unknown. Here, we found that Cas1, a highly conserved component known to exclusively work in the adaptation stage, also mediates the interference stage through connecting Cas2/3 to the DNA-bound CRISPR-associated complex for antiviral defense (Cascade; CRISPR system yersinia, Csy) of the ICP1 CRISPR-Cas system. A series of structures of Csy, Csy-dsDNA (double-stranded DNA), Cas1-Cas2/3 and Csy-dsDNA-Cas1-Cas2/3 complexes reveal the whole process of Cas1-mediated target DNA cleavage by the ICP1 CRISPR-Cas system. Together, these data support an unprecedented model in which Cas1 mediates the interference stage in a phage-encoded CRISPR-Cas system and the study also sheds light on a unique model of primed adaptation.

PubMed: 38977786
DOI: 10.1038/s41589-024-01659-5

Experimental method

X-RAY DIFFRACTION (2.995 Å)