8JZN

Structure of a fungal 1,3-beta-glucan synthase

Summary for 8JZN

• Entry DOI: 10.2210/pdb8jzn/pdb
• EMDB information: 36748
• Descriptor: 1,3-beta-glucan synthase component FKS1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate, ... (5 entities in total)
• Functional Keywords: transferase, fungi, drug target
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Total number of polymer chains: 1
• Total formula weight: 224584.16

Authors

Zhao, C.,You, Z.,Chen, D.,Hang, J.,Wang, Z.,Meng, J.,Wang, L.,Zhao, P.,Qiao, J.,Yun, C.,Bai, L. (deposition date: 2023-07-05, release date: 2023-10-04, Last modification date: 2025-06-25)

Primary citation

Zhao, C.R.,You, Z.L.,Chen, D.D.,Hang, J.,Wang, Z.B.,Ji, M.,Wang, L.X.,Zhao, P.,Qiao, J.,Yun, C.H.,Bai, L.
Structure of a fungal 1,3-beta-glucan synthase.
Sci Adv, 9:eadh7820-eadh7820, 2023

PubMed Abstract: 1,3-β-Glucan serves as the primary component of the fungal cell wall and is produced by 1,3-β-glucan synthase located in the plasma membrane. This synthase is a molecular target for antifungal drugs such as echinocandins and the triterpenoid ibrexafungerp. In this study, we present the cryo-electron microscopy structure of 1,3-β-glucan synthase (Fks1) at 2.47-Å resolution. The structure reveals a central catalytic region adopting a cellulose synthase fold with a cytosolic conserved GT-A-type glycosyltransferase domain and a closed transmembrane channel responsible for glucan transportation. Two extracellular disulfide bonds are found to be crucial for Fks1 enzymatic activity. Through structural comparative analysis with cellulose synthases and structure-guided mutagenesis studies, we gain previously unknown insights into the molecular mechanisms of fungal 1,3-β-glucan synthase.

PubMed: 37703377
DOI: 10.1126/sciadv.adh7820

Experimental method

ELECTRON MICROSCOPY (2.47 Å)