8JJ6

Structure of the NELF-BCE complex

Summary for 8JJ6

• Entry DOI: 10.2210/pdb8jj6/pdb
• Descriptor: Negative elongation factor B, Negative elongation factor complex member C/D, NELF-E, ... (4 entities in total)
• Functional Keywords: transcription elongation factor, negative transcription elongation factor(nelf), transcription
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 6
• Total formula weight: 172885.60

Authors

Wang, Z.,Cao, Y.,Qin, Y. (deposition date: 2023-05-29, release date: 2023-08-30, Last modification date: 2024-09-11)

Primary citation

Cao, Y.,Qin, Y.,Zhang, W.,Tian, W.,Ren, Y.,Ren, J.,Wang, J.,Wang, M.,Jiang, J.,Wang, Z.
Structural basis of the human negative elongation factor NELF-B/C/E ternary complex.
Biochem.Biophys.Res.Commun., 677:155-161, 2023

PubMed Abstract: Negative elongation factor (NELF) is a four-subunit transcription elongation factor that mainly functions in maintaining the paused state of RNA polymerase II in eukaryotes. Upon binding to Pol II, NELF works synergistically with DRB sensitivity-inducing factor (DSIF) and inhibits transcription elongation of Pol II, which subsequently retains a stably paused state 20-60 base pairs downstream of the promoter. The promoter-proximal pausing of Pol II caused by NELF is a general mechanism of transcriptional regulation for most signal-responsive genes. To date, structural studies have significantly advanced our understanding of the molecular mechanisms of NELF. However, a high quality structural model clarifying the interaction details of this complex is still lacking. In this study, we solved the high resolution crystal structure of the NELF-B/C/E ternary complex. We observed detailed interactions between subunits and identified residues important for the association between NELF-B and NELF-E. Our work presents a precise model of the NELF complex, which will facilitate our understanding of its in vivo function.

PubMed: 37591184
DOI: 10.1016/j.bbrc.2023.08.019

Experimental method

X-RAY DIFFRACTION (2.72 Å)