8JIW

Atomic structure of wheat ribosome reveals unique features of the plant ribosomes

This is a non-PDB format compatible entry.

Summary for 8JIW

• Entry DOI: 10.2210/pdb8jiw/pdb
• EMDB information: 36331 36332
• Descriptor: 18S rRNA, 40S ribosomal protein S11 N-terminal domain-containing protein, Ribosomal protein S13/S15 N-terminal domain-containing protein, ... (36 entities in total)
• Functional Keywords: protein synthesis machinery, eukaryotic ribosome, plant, translation
• Biological source: Triticum aestivum (bread wheat); More
• Total number of polymer chains: 33
• Total formula weight: 1178903.91

Authors

Mishra, R.K.,Sharma, P.,Hussain, T. (deposition date: 2023-05-28, release date: 2024-03-27, Last modification date: 2024-05-22)

Primary citation

Mishra, R.K.,Sharma, P.,Khaja, F.T.,Uday, A.B.,Hussain, T.
Cryo-EM structure of wheat ribosome reveals unique features of the plant ribosomes.
Structure, 32:562-, 2024

PubMed Abstract: Plants being sessile organisms exhibit unique features in ribosomes, which might aid in rapid gene expression and regulation in response to varying environmental conditions. Here, we present high-resolution structures of the 60S and 80S ribosomes from wheat, a monocot staple crop plant (Triticum aestivum). While plant ribosomes have unique plant-specific rRNA modification (Cm1847) in the peptide exit tunnel (PET), the zinc-finger motif in eL34 is absent, and uL4 is extended, making an exclusive interaction network. We note differences in the eL15-helix 11 (25S) interaction, eL6-ES7 assembly, and certain rRNA chemical modifications between monocot and dicot ribosomes. In eukaryotes, we observe highly conserved rRNA modification (Gm75) in 5.8S rRNA and a flipped base (G1506) in PET. These features are likely involved in sensing or stabilizing nascent chain. Finally, we discuss the importance of the universal conservation of three consecutive rRNA modifications in all ribosomes for their interaction with A-site aminoacyl-tRNA.

PubMed: 38458197
DOI: 10.1016/j.str.2024.02.006

Experimental method

ELECTRON MICROSCOPY (2.88 Å)