8JIV

Atomic structure of wheat ribosome reveals unique features of the plant ribosomes

これはPDB形式変換不可エントリーです。

8JIV の概要

• エントリーDOI: 10.2210/pdb8jiv/pdb
• EMDBエントリー: 36331
• 分子名称: 25S rRNA, 60S ribosomal protein L7a, Ribosomal protein L6 alpha-beta domain-containing protein, ... (47 entities in total)
• 機能のキーワード: protein synthesis machinery, eukaryotic ribosome, plant, translation, ribosome
• 由来する生物種: Triticum aestivum (bread wheat); 詳細
• タンパク質・核酸の鎖数: 44
• 化学式量合計: 1984466.28

構造登録者

Mishra, R.K.,Sharma, P.,Hussain, T. (登録日: 2023-05-28, 公開日: 2024-03-27, 最終更新日: 2024-05-22)

主引用文献

Mishra, R.K.,Sharma, P.,Khaja, F.T.,Uday, A.B.,Hussain, T.
Cryo-EM structure of wheat ribosome reveals unique features of the plant ribosomes.
Structure, 32:562-, 2024

PubMed Abstract: Plants being sessile organisms exhibit unique features in ribosomes, which might aid in rapid gene expression and regulation in response to varying environmental conditions. Here, we present high-resolution structures of the 60S and 80S ribosomes from wheat, a monocot staple crop plant (Triticum aestivum). While plant ribosomes have unique plant-specific rRNA modification (Cm1847) in the peptide exit tunnel (PET), the zinc-finger motif in eL34 is absent, and uL4 is extended, making an exclusive interaction network. We note differences in the eL15-helix 11 (25S) interaction, eL6-ES7 assembly, and certain rRNA chemical modifications between monocot and dicot ribosomes. In eukaryotes, we observe highly conserved rRNA modification (Gm75) in 5.8S rRNA and a flipped base (G1506) in PET. These features are likely involved in sensing or stabilizing nascent chain. Finally, we discuss the importance of the universal conservation of three consecutive rRNA modifications in all ribosomes for their interaction with A-site aminoacyl-tRNA.

PubMed: 38458197
DOI: 10.1016/j.str.2024.02.006

実験手法

ELECTRON MICROSCOPY (2.84 Å)