8JIB

Crystal Structure of Prophenoloxidase PPO6 from Aedes aegypti

8JIB の概要

• エントリーDOI: 10.2210/pdb8jib/pdb
• 分子名称: TK receptor, COPPER (II) ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: prophenoloxidase., immune system
• 由来する生物種: Aedes aegypti (yellow fever mosquito)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 956361.02

構造登録者

Zhu, X.,Zhang, L.,Yang, X.,Bao, P.,Ren, D.,Han, Q. (登録日: 2023-05-26, 公開日: 2023-11-29, 最終更新日: 2025-04-02)

主引用文献

Zhu, X.,Zhang, L.,Jiang, L.,Chen, H.,Tang, Y.,Yang, X.,Bao, P.,Liao, C.,Li, J.,Vavricka, C.J.,Ren, D.,Chen, Z.,Guo, Y.,Han, Q.
The Aedes aegypti mosquito evolves two types of prophenoloxidases with diversified functions.
Proc.Natl.Acad.Sci.USA, 122:e2413131122-e2413131122, 2025

PubMed Abstract: Insect phenoloxidase, presented as an inactive precursor prophenoloxidase (PPO) in hemolymph, catalyzes melanin formation, which is involved in wound healing, pathogen killing, reversible oxygen collection during insect respiration, and cuticle and eggshell formation. Mosquitoes possess 9 to 16 PPO members across different genera, a number that is more than that found in other dipteran insects. However, the reasons for the redundancy of these PPOs and whether they have distinct biochemical properties and physiological functions remain unclear. Phylogenetic analysis confirmed that PPO6 (Aea-PPO6) is an ortholog to PPOs in other insect species, classified as the classical insect type, while other Aea-PPOs are unique to Diptera, herein referred to as the dipteran type here. We characterized two Aea-PPO members, Aea-PPO6, the classical insect type, and Aea-PPO10, a dipteran type, which exhibit distinct substrate specificities. By resolving Aea-PPO6's crystal structure and creating a chimera protein (Aea-PPO6-cm) with Motif 1 (GDGPDSVVR) from Aea-PPO10, we identified the motif that determines PPO substrate specificity. In vivo, loss of Aea-PPO6 led to larval lethality, while Aea-PPO10 was involved in development, pigmentation, and immunity. Our results enhance the understanding of the functional diversification of mosquito PPOs.

PubMed: 39808654
DOI: 10.1073/pnas.2413131122

実験手法

X-RAY DIFFRACTION (3.15 Å)