8JDR
Crystal structure of H405A mLDHD in complex with D-2-hydroxy-3-methyl-valeric acid
Summary for 8JDR
| Entry DOI | 10.2210/pdb8jdr/pdb |
| Descriptor | Probable D-lactate dehydrogenase, mitochondrial, FLAVIN-ADENINE DINUCLEOTIDE, (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid, ... (4 entities in total) |
| Functional Keywords | d-lactate dehydrogenase, ldhd, 2-hydroxyacid, oxidoreductase |
| Biological source | Mus musculus (house mouse) |
| Total number of polymer chains | 1 |
| Total formula weight | 51303.50 |
| Authors | |
| Primary citation | Jin, S.,Chen, X.,Yang, J.,Ding, J. Lactate dehydrogenase D is a general dehydrogenase for D-2-hydroxyacids and is associated with D-lactic acidosis. Nat Commun, 14:6638-6638, 2023 Cited by PubMed Abstract: Mammalian lactate dehydrogenase D (LDHD) catalyzes the oxidation of D-lactate to pyruvate. LDHD mutations identified in patients with D-lactic acidosis lead to deficient LDHD activity. Here, we perform a systematic biochemical study of mouse LDHD (mLDHD) and determine the crystal structures of mLDHD in FAD-bound form and in complexes with FAD, Mn and a series of substrates or products. We demonstrate that mLDHD is an Mn-dependent general dehydrogenase which exhibits catalytic activity for D-lactate and other D-2-hydroxyacids containing hydrophobic moieties, but no activity for their L-isomers or D-2-hydroxyacids containing hydrophilic moieties. The substrate-binding site contains a positively charged pocket to bind the common glycolate moiety and a hydrophobic pocket with some elasticity to bind the varied hydrophobic moieties of substrates. The structural and biochemical data together reveal the molecular basis for the substrate specificity and catalytic mechanism of LDHD, and the functional roles of mutations in the pathogenesis of D-lactic acidosis. PubMed: 37863926DOI: 10.1038/s41467-023-42456-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.64 Å) |
Structure validation
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