8J7Z

Structure of FCP trimer in Cyclotella meneghiniana

Summary for 8J7Z

• Entry DOI: 10.2210/pdb8j7z/pdb
• EMDB information: 36054
• Descriptor: FCP, (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'- yl acetate, CHLOROPHYLL A, ... (6 entities in total)
• Functional Keywords: cm-fcp-trimer, photosynthesis
• Biological source: Stephanocyclus meneghinianus
• Total number of polymer chains: 3
• Total formula weight: 102598.84

Authors

Shen, L.L.,Li, Z.H.,Shen, J.R.,Wang, W.D. (deposition date: 2023-04-28, release date: 2023-12-20, Last modification date: 2025-06-18)

Primary citation

Zhao, S.,Shen, L.,Li, X.,Tao, Q.,Li, Z.,Xu, C.,Zhou, C.,Yang, Y.,Sang, M.,Han, G.,Yu, L.J.,Kuang, T.,Shen, J.R.,Wang, W.
Structural insights into photosystem II supercomplex and trimeric FCP antennae of a centric diatom Cyclotella meneghiniana.
Nat Commun, 14:8164-8164, 2023

PubMed Abstract: Diatoms are dominant marine algae and contribute around a quarter of global primary productivity, the success of which is largely attributed to their photosynthetic capacity aided by specific fucoxanthin chlorophyll-binding proteins (FCPs) to enhance the blue-green light absorption under water. We purified a photosystem II (PSII)-FCPII supercomplex and a trimeric FCP from Cyclotella meneghiniana (Cm) and solved their structures by cryo-electron microscopy (cryo-EM). The structures reveal detailed organizations of monomeric, dimeric and trimeric FCP antennae, as well as distinct assemblies of Lhcx6_1 and dimeric FCPII-H in PSII core. Each Cm-PSII-FCPII monomer contains an Lhcx6_1, an FCP heterodimer and other three FCP monomers, which form an efficient pigment network for harvesting energy. More diadinoxanthins and diatoxanthins are found in FCPs, which may function to quench excess energy. The trimeric FCP contains more chlorophylls c and fucoxanthins. These diversified FCPs and PSII-FCPII provide a structural basis for efficient light energy harvesting, transfer, and dissipation in C. meneghiniana.

PubMed: 38071196
DOI: 10.1038/s41467-023-44055-8

Experimental method

ELECTRON MICROSCOPY (2.72 Å)