8J6T

Cryo-EM structure of the double CAF-1 bound right-handed Di-tetrasome

Summary for 8J6T

• Entry DOI: 10.2210/pdb8j6t/pdb
• EMDB information: 36014
• Descriptor: Histone H3.1, Histone H4, Widom 601 DNA (147-MER), ... (6 entities in total)
• Functional Keywords: replication-dna complex, replication/dna
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 14
• Total formula weight: 535371.51

Authors

Liu, C.P.,Yu, Z.Y.,Xu, R.M. (deposition date: 2023-04-26, release date: 2023-08-16, Last modification date: 2023-09-06)

Primary citation

Liu, C.P.,Yu, Z.,Xiong, J.,Hu, J.,Song, A.,Ding, D.,Yu, C.,Yang, N.,Wang, M.,Yu, J.,Hou, P.,Zeng, K.,Li, Z.,Zhang, Z.,Zhang, X.,Li, W.,Zhang, Z.,Zhu, B.,Li, G.,Xu, R.M.
Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1.
Science, 381:eadd8673-eadd8673, 2023

PubMed Abstract: Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly process is lacking. In this work, we report the crystal structure of human CAF-1 in the absence of histones and the cryo-electron microscopy structure of CAF-1 in complex with histones H3 and H4. One histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the acidic domain of the p150 subunit. We also observed a dimeric CAF-1-H3-H4 supercomplex in which two H3-H4 heterodimers are poised for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tetramers. These findings signify the involvement of DNA in H3-H4 tetramer formation and suggest a right-handed nucleosome precursor in chromatin replication.

PubMed: 37616371
DOI: 10.1126/science.add8673

Experimental method

ELECTRON MICROSCOPY (6.6 Å)