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8J60

Structural and mechanistic insight into ribosomal ITS2 RNA processing by nuclease-kinase machinery

8J60 の概要
エントリーDOI10.2210/pdb8j60/pdb
EMDBエントリー33735
分子名称Polynucleotide 5'-hydroxyl-kinase GRC3, LAS1 protein (2 entities in total)
機能のキーワードendoribonuclease; ribosome biosynthesis; las1; grc3, rna binding protein
由来する生物種Cyberlindnera jadinii
詳細
タンパク質・核酸の鎖数4
化学式量合計238052.98
構造登録者
Chen, J.,Chen, H.,Li, S.,Lin, X.,Hu, R.,Zhang, K.,Liu, L. (登録日: 2023-04-24, 公開日: 2024-01-17, 最終更新日: 2024-10-16)
主引用文献Chen, J.,Chen, H.,Li, S.,Lin, X.,Hu, R.,Zhang, K.,Liu, L.
Structural and mechanistic insights into ribosomal ITS2 RNA processing by nuclease-kinase machinery.
Elife, 12:-, 2024
Cited by
PubMed Abstract: Precursor ribosomal RNA (pre-rRNA) processing is a key step in ribosome biosynthesis and involves numerous RNases. A HEPN (higher eukaryote and prokaryote nucleotide binding) nuclease Las1 and a polynucleotide kinase Grc3 assemble into a tetramerase responsible for rRNA maturation. Here, we report the structures of full-length and Las1-Grc3 complexes, and Las1. The Las1-Grc3 structures show that the central coiled-coil domain of Las1 facilitates pre-rRNA binding and cleavage, while the Grc3 C-terminal loop motif directly binds to the HEPN active center of Las1 and regulates pre-rRNA cleavage. Structural comparison between Las1 and Las1-Grc3 complex exhibits that Grc3 binding induces conformational rearrangements of catalytic residues associated with HEPN nuclease activation. Biochemical assays identify that Las1 processes pre-rRNA at the two specific sites (C2 and C2'), which greatly facilitates rRNA maturation. Our structures and specific pre-rRNA cleavage findings provide crucial insights into the mechanism and pathway of pre-rRNA processing in ribosome biosynthesis.
PubMed: 38180340
DOI: 10.7554/eLife.86847
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (3.39 Å)
構造検証レポート
Validation report summary of 8j60
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-04-02に公開中

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