8J59
The structure of a novel thermophilic-like old yellow enzyme from Aspergillus flavus-AfOYE1
Summary for 8J59
| Entry DOI | 10.2210/pdb8j59/pdb |
| Descriptor | NADPH dehydrogenase afvA, FLAVIN MONONUCLEOTIDE, CHLORIDE ION, ... (7 entities in total) |
| Functional Keywords | thermophilic-like, old yellow enzyme, alkenes, enantioselectivity, biosynthetic protein |
| Biological source | Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) |
| Total number of polymer chains | 2 |
| Total formula weight | 86308.60 |
| Authors | |
| Primary citation | Li, N.,Wang, Y.,Meng, Y.,Lv, Y.,Zhang, S.,Wei, S.,Ma, P.,Hu, Y.,Lin, H. Structural and functional characterization of a new thermophilic-like OYE from Aspergillus flavus. Appl.Microbiol.Biotechnol., 108:134-134, 2024 Cited by PubMed Abstract: Old yellow enzymes (OYEs) have been proven as powerful biocatalysts for the asymmetric reduction of activated alkenes. Fungi appear to be valuable sources of OYEs, but most of the fungal OYEs are unexplored. To expand the OYEs toolbox, a new thermophilic-like OYE (AfOYE1) was identified from Aspergillus flavus strain NRRL3357. The thermal stability analysis showed that the T of AfOYE1 was 60 °C, and it had the optimal temperature at 45 °C. Moreover, AfOYE1 exhibited high reduction activity in a wide pH range (pH 5.5-8.0). AfOYE1 could accept cyclic enones, acrylamide, nitroalkenes, and α, β-unsaturated aldehydes as substrates and had excellent enantioselectivity toward prochiral alkenes (> 99% ee). Interestingly, an unexpected (S)-stereoselectivity bioreduction toward 2-methylcyclohexenone was observed. The further crystal structure of AfOYE1 revealed that the "cap" region from Ala132 to Thr182, the loop of Ser316 to Gly325, α short helix of Arg371 to Gln375, and the C-terminal "finger" structure endow the catalytic cavity of AfOYE1 quite deep and narrow, and flavin mononucleotide (FMN) heavily buried at the bottom of the active site tunnel. Furthermore, the catalytic mechanism of AfOYE1 was also investigated, and the results confirmed that the residues His211, His214, and Tyr216 compose its catalytic triad. This newly identified thermophilic-like OYE would thus be valuable for asymmetric alkene hydrogenation in industrial processes. KEY POINTS: A new thermophilic-like OYE AfOYE1 was identified from Aspergillus flavus, and the T of AfOYE1 was 60 °C AfOYE1 catalyzed the reduction of 2-methylcyclohexenone with (S)-stereoselectivity The crystal structure of AfOYE1 was revealedv. PubMed: 38229304DOI: 10.1007/s00253-023-12963-w PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.55 Å) |
Structure validation
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