8J2G

Human ZnT1-D47N/D255N mutant

Summary for 8J2G

• Entry DOI: 10.2210/pdb8j2g/pdb
• EMDB information: 35949
• Descriptor: Proton-coupled zinc antiporter SLC30A1 (1 entity in total)
• Functional Keywords: zinc exporter, transport protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 110721.27

Authors

Sun, L.,Liu, X.,Sun, C.,He, B. (deposition date: 2023-04-14, release date: 2024-04-17, Last modification date: 2024-10-30)

Primary citation

Sun, C.,He, B.,Gao, Y.,Wang, X.,Liu, X.,Sun, L.
Structural insights into the calcium-coupled zinc export of human ZnT1.
Sci Adv, 10:eadk5128-eadk5128, 2024

PubMed Abstract: Cellular zinc (Zn) homeostasis is essential to human health and is under tight regulations. Human zinc transporter 1 (hZnT1) is a plasma membrane-localized Zn exporter belonging to the ZnT family, and its functional aberration is associated with multiple diseases. Here, we show that hZnT1 works as a Zn/Ca exchanger. We determine the structure of hZnT1 using cryo-electron microscopy (cryo-EM) single particle analysis. hZnT1 adopts a homodimeric structure, and each subunit contains a transmembrane domain consisting of six transmembrane segments, a cytosolic domain, and an extracellular domain. The transmembrane region displays an outward-facing conformation. On the basis of structural and functional analysis, we propose a model for the hZnT1-mediated Zn/Ca exchange. Together, these results facilitate our understanding of the biological functions of hZnT1 and provide a basis for further investigations of the ZnT family transporters.

PubMed: 38669333
DOI: 10.1126/sciadv.adk5128

Experimental method

ELECTRON MICROSCOPY (3.4 Å)