8J1M

Crystal structure of a crosslinked variant of BbZIP

Summary for 8J1M

• Entry DOI: 10.2210/pdb8j1m/pdb
• Descriptor: Putative membrane protein, CADMIUM ION, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: transporter, zinc, cadmium, mercury, crosslink, membrane protein
• Biological source: Bordetella bronchiseptica RB50
• Total number of polymer chains: 1
• Total formula weight: 31328.51

Authors

Zhang, T.,Zhang, Y.,Sui, D.,Hu, J. (deposition date: 2023-04-13, release date: 2024-11-13)

Primary citation

Zhang, Y.,Jafari, M.,Zhang, T.,Sui, D.,Sagresti, L.,Merz Jr., K.M.,Hu, J.
Molecular insights into substrate translocation in an elevator-type metal transporter.
Biorxiv, 2024

PubMed Abstract: The Zrt/Irt-like protein (ZIP) metal transporters are key players in maintaining the homeostasis of a panel of essential microelements. The prototypical ZIP from (BbZIP) is an elevator transporter, but how the metal substrate moves along the transport pathway and how the transporter changes conformation to allow alternating access remain to be elucidated. Here, we combined structural, biochemical, and computational approaches to investigate the process of metal substrate translocation along with the global structural rearrangement. Our study revealed an upward hinge motion of the transport domain in a high-resolution crystal structure of a cross-linked variant, elucidated the mechanisms of metal release from the transport site into the cytoplasm and activity regulation by a cytoplasmic metal-binding loop, and unraveled an unusual elevator mode in enhanced sampling simulations that distinguishes BbZIP from other elevator transporters. This work provides important insights into the metal transport mechanism of the ZIP family.

PubMed: 39345646
DOI: 10.1101/2024.09.18.613805

Experimental method

X-RAY DIFFRACTION (1.952 Å)