8IXK

bottom segment of the bacteriophage M13 mini variant

Summary for 8IXK

• Entry DOI: 10.2210/pdb8ixk/pdb
• EMDB information: 35794
• Descriptor: Attachment protein G3P, Capsid protein G8P, Head virion protein G6P (3 entities in total)
• Functional Keywords: viral coat protein, m13, viral protein
• Biological source: Inovirus M13; More
• Total number of polymer chains: 25
• Total formula weight: 389154.29

Authors

Xiang, Y.,Jia, Q. (deposition date: 2023-04-01, release date: 2023-09-13, Last modification date: 2023-09-20)

Primary citation

Jia, Q.,Xiang, Y.
Cryo-EM structure of a bacteriophage M13 mini variant.
Nat Commun, 14:5421-5421, 2023

PubMed Abstract: Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages.

PubMed: 37669979
DOI: 10.1038/s41467-023-41151-7

Experimental method

ELECTRON MICROSCOPY (3.3 Å)