8ITE

Crystal structure of pE301R from African swine fever virus

Replaces:  7DRH

Summary for 8ITE

• Entry DOI: 10.2210/pdb8ite/pdb
• Descriptor: Uncharacterized protein E301R (2 entities in total)
• Functional Keywords: pcna-like protein, dna binding protein
• Biological source: African swine fever virus
• Total number of polymer chains: 1
• Total formula weight: 35299.58

Authors

Zhang, H.,Li, Y.H. (deposition date: 2023-03-22, release date: 2023-09-13, Last modification date: 2024-03-27)

Primary citation

Li, S.,Ge, H.,Li, Y.,Zhang, K.,Yu, S.,Cao, H.,Wang, Y.,Deng, H.,Li, J.,Dai, J.,Li, L.F.,Luo, Y.,Sun, Y.,Geng, Z.,Dong, Y.,Zhang, H.,Qiu, H.J.
The E301R protein of African swine fever virus functions as a sliding clamp involved in viral genome replication.
Mbio, 14:e0164523-e0164523, 2023

PubMed Abstract: Sliding clamp is a highly conserved protein in the evolution of prokaryotic and eukaryotic cells. The sliding clamp is required for genomic replication as a critical co-factor of DNA polymerases. However, the sliding clamp analogs in viruses remain largely unknown. We found that the ASFV E301R protein (pE301R) exhibited a sliding clamp-like structure and similar functions during ASFV replication. Interestingly, pE301R is assembled into a unique ring-shaped homotetramer distinct from sliding clamps or proliferating cell nuclear antigens (PCNAs) from other species. Notably, the gene is required for viral life cycle, but the pE301R function can be partially restored by the porcine PCNA. This study not only highlights the functional role of the ASFV pE301R as a viral sliding clamp analog, but also facilitates the dissection of the complex replication mechanism of ASFV, which provides novel clues for developing antivirals against ASF.

PubMed: 37772878
DOI: 10.1128/mbio.01645-23

Experimental method

X-RAY DIFFRACTION (2.1 Å)