8IRJ

Cryo-EM structure of an orphan GPCR

Summary for 8IRJ

• Entry DOI: 10.2210/pdb8irj/pdb
• EMDB information: 35676
• Descriptor: Probable G-protein coupled receptor 179 (1 entity in total)
• Functional Keywords: gpcr, signaling protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 167917.55

Authors

Yun, Y.,Jeong, H.,Lee, H.H. (deposition date: 2023-03-17, release date: 2024-09-18, Last modification date: 2025-10-01)

Primary citation

Yun, Y.,Jeong, H.,Laboute, T.,Martemyanov, K.A.,Lee, H.H.
Cryo-EM structure of human class C orphan GPCR GPR179 involved in visual processing.
Nat Commun, 15:8299-8299, 2024

PubMed Abstract: GPR179, an orphan class C GPCR, is expressed at the dendritic tips of ON-bipolar cells in the retina. It plays a pivotal role in the initial synaptic transmission of visual signals from photoreceptors, and its deficiency is known to be the cause of complete congenital stationary night blindness. Here, we present the cryo-electron microscopy structure of human GPR179. Notably, the transmembrane domain (TMD) of GPR179 forms a homodimer through the TM1/7 interface with a single inter-protomer disulfide bond, adopting a noncanonical dimerization mode. Furthermore, the TMD dimer exhibits architecture well-suited for the highly curved membrane of the dendritic tip and distinct from the flat membrane arrangement observed in other class C GPCR dimers. Our structure reveals unique structural features of GPR179 TMD, setting it apart from other class C GPCRs. These findings provide a foundation for understanding signal transduction through GPR179 in visual processing and offers insights into the underlying causes of ocular diseases.

PubMed: 39333506
DOI: 10.1038/s41467-024-52584-z

Experimental method

ELECTRON MICROSCOPY (3.49 Å)