8IPP
Crystal structure of the complex between an ankyrin and a parallel G-quadruplex
Summary for 8IPP
| Entry DOI | 10.2210/pdb8ipp/pdb |
| Related | 8IPF |
| Descriptor | 93del-T12, DARPin protein 2E4, POTASSIUM ION, ... (5 entities in total) |
| Functional Keywords | g-quadruplex, darpins, parallel, dna binding protein-dna complex, dna binding protein/dna |
| Biological source | synthetic construct More |
| Total number of polymer chains | 2 |
| Total formula weight | 20161.89 |
| Authors | |
| Primary citation | Ngo, K.H.,Liew, C.W.,Heddi, B.,Phan, A.T. Structural Basis for Parallel G-Quadruplex Recognition by an Ankyrin Protein. J.Am.Chem.Soc., 146:13709-13713, 2024 Cited by PubMed Abstract: G-Quadruplex (G4) structures formed by guanine-rich DNA and RNA sequences are implicated in various biological processes. Understanding the mechanisms by which proteins recognize G4 structures is crucial for elucidating their functional roles. Here we present the X-ray crystal structure of an ankyrin protein bound to a parallel G4 structure. Our findings reveal a new specific recognition mode in which a bundle of α-helices and loops of the ankyrin form a flat surface to stack on the G-tetrad core. The protein employs a combination of hydrogen bonds and hydrophobic contacts to interact with the G4, and electrostatic interaction is used to enhance the binding affinity. This binding mechanism provides valuable insights into understanding G4 recognition by proteins. PubMed: 38738955DOI: 10.1021/jacs.4c01971 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.007 Å) |
Structure validation
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