8IKJ

Cryo-EM structure of the inactive CD97

Summary for 8IKJ

• Entry DOI: 10.2210/pdb8ikj/pdb
• EMDB information: 35514
• Descriptor: Adhesion G protein-coupled receptor E5,Soluble cytochrome b562,Adhesion G protein-coupled receptor E5 subunit beta, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• Functional Keywords: adhension gpcr, cd97, inactive, membrane protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 1
• Total formula weight: 73215.28

Authors

Mao, C.,Zhao, R.,Dong, Y.,Gao, M.,Chen, L.,Zhang, C.,Xiao, P.,Guo, J.,Qin, J.,Shen, D.,Ji, S.,Zang, S.,Zhang, H.,Wang, W.,Shen, Q.,Sun, P.,Zhang, Y. (deposition date: 2023-02-28, release date: 2024-02-14, Last modification date: 2024-10-30)

Primary citation

Mao, C.,Zhao, R.J.,Dong, Y.J.,Gao, M.,Chen, L.N.,Zhang, C.,Xiao, P.,Guo, J.,Qin, J.,Shen, D.D.,Ji, S.Y.,Zang, S.K.,Zhang, H.,Wang, W.W.,Shen, Q.,Sun, J.P.,Zhang, Y.
Conformational transitions and activation of the adhesion receptor CD97.
Mol.Cell, 84:570-583.e7, 2024

PubMed Abstract: Adhesion G protein-coupled receptors (aGPCRs) are evolutionarily ancient receptors involved in a variety of physiological and pathophysiological processes. Modulators of aGPCR, particularly antagonists, hold therapeutic promise for diseases like cancer and immune and neurological disorders. Hindered by the inactive state structural information, our understanding of antagonist development and aGPCR activation faces challenges. Here, we report the cryo-electron microscopy structures of human CD97, a prototypical aGPCR that plays crucial roles in immune system, in its inactive apo and G13-bound fully active states. Compared with other family GPCRs, CD97 adopts a compact inactive conformation with a constrained ligand pocket. Activation induces significant conformational changes for both extracellular and intracellular sides, creating larger cavities for Stachel sequence binding and G13 engagement. Integrated with functional and metadynamics analyses, our study provides significant mechanistic insights into the activation and signaling of aGPCRs, paving the way for future drug discovery efforts.

PubMed: 38215752
DOI: 10.1016/j.molcel.2023.12.020

Experimental method

ELECTRON MICROSCOPY (3.2 Å)