8IHW

X-ray crystal structure of D43R mutant of endo-1,4-beta glucanase from Eisenia fetida

Summary for 8IHW

Entry DOI	10.2210/pdb8ihw/pdb
Descriptor	Endoglucanase, GLYCEROL, MAGNESIUM ION, ... (5 entities in total)
Functional Keywords	earthworm, cellulase, cold-adapted enzyme, hydrolase
Biological source	Eisenia fetida (common brandling worm)
Total number of polymer chains	1
Total formula weight	51903.67
Authors	Kuroki, C.,Hirano, Y.,Nakazawa, M.,Sakamoto, T.,Tamada, T.,Ueda, M. (deposition date: 2023-02-24, release date: 2023-12-06)
Primary citation	Kuroki, C.,Hirano, Y.,Nakazawa, M.,Sakamoto, T.,Tamada, T.,Ueda, M. A single mutation Asp43Arg was increased 2.5-fold the catalytic activity and maintained the stability of cold-adapted endo-1,4-beta glucanase (Ef-EG2) from Eisenia fetida. Curr Res Biotechnol, 5:-, 2023 Cited by DOI: 10.1016/j.crbiot.2023.100126 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation