8IEX

Solution structure of AtWRKY11-DBD

Summary for 8IEX

• Entry DOI: 10.2210/pdb8iex/pdb
• NMR Information: BMRB: 36548
• Descriptor: Probable WRKY transcription factor 11, ZINC ION (2 entities in total)
• Functional Keywords: transcriptional factor, dna binding protein
• Biological source: Arabidopsis thaliana (Mouse-ear cress)
• Total number of polymer chains: 1
• Total formula weight: 11090.11

Authors

Dong, X.,Hu, Y.F. (deposition date: 2023-02-16, release date: 2024-02-21, Last modification date: 2024-09-25)

Primary citation

Wang, J.,Lin, Y.,Yang, J.,Zhang, Q.,Liu, M.,Hu, Y.,Dong, X.
Solution structure of the DNA binding domain of Arabidopsis transcription factor WRKY11.
Biochem.Biophys.Res.Commun., 653:133-139, 2023

PubMed Abstract: The Arabidopsis WRKY11 (AtWRKY11) protein is an important transcription factor involved in plant response to biotic and abiotic stresses. Its DNA-binding domain specifically binds to gene promoter regions harboring the W-box consensus motif. Herein we report the high-resolution structure of the AtWRKY11 DNA-binding domain (DBD) determined by solution NMR spectroscopy. The results show that AtWRKY11-DBD adopts an all-β fold comprising five β-strands packed in an antiparallel topology, stabilized by a zinc-finger motif. Structural comparison reveals that the long β-β loop shows the highest structural variation from other available WRKY domain structures. Moreover, this loop was further found to contribute to the binding between AtWRKY11-DBD and W-box DNA. Our current study provides atomic-level structural basis for further understanding the structure-function relationship of plant WRKY proteins.

PubMed: 36868077
DOI: 10.1016/j.bbrc.2023.02.072

Experimental method

SOLUTION NMR