8IBM

Sulfate bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation

Summary for 8IBM

• Entry DOI: 10.2210/pdb8ibm/pdb
• Descriptor: Alpha/beta hydrolase family protein, CALCIUM ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: protein engineering, polyesterase, disulfide bond, metal binding, ligand complex, hydrolase
• Biological source: Saccharomonospora viridis
• Total number of polymer chains: 2
• Total formula weight: 58051.36

Authors

Emori, M.,Numoto, N.,Kamiya, N.,Oda, M. (deposition date: 2023-02-10, release date: 2023-03-15)

• Primary citation: Numoto, N.,Kamiya, N.,Oda, M.; Improvement of thermostability and activity of PET-degrading enzyme Cut190 towards a detailed understanding and application of the enzymatic reaction mechanism.; Biorxiv, 2023; DOI: 10.1101/2023.02.26.529345

Experimental method

X-RAY DIFFRACTION (2.2 Å)