8I7P

Crystal structure of Ricin A chain bound with N2-(2-amino-4-oxo-3,4-dihydropteridine-7-carbonyl)glycyl-L-tyrosine

Summary for 8I7P

• Entry DOI: 10.2210/pdb8i7p/pdb
• Descriptor: Ricin A chain, 6-(2-ethyl-4-hydroxyphenyl)-1H-indazole-3-carboxamide, SULFATE ION, ... (4 entities in total)
• Functional Keywords: toxin, inhibitor, hydrolase
• Biological source: Ricinus communis (castor bean); More
• Total number of polymer chains: 2
• Total formula weight: 31516.31

Authors

Goto, M.,Sakamoto, N.,Higashi, S.,Kawata, R.,Nagatsu, K.,Saito, R. (deposition date: 2023-02-01, release date: 2023-09-20, Last modification date: 2024-10-09)

Primary citation

Goto, M.,Sakamoto, N.,Higashi, S.,Kawata, R.,Nagatsu, K.,Saito, R.
Crystal structure of ricin toxin A chain complexed with a highly potent pterin-based small-molecular inhibitor.
J Enzyme Inhib Med Chem, 38:2219038-2219038, 2023

PubMed Abstract: Ricin toxin A chain (RTA), from , is a deadly protein that inactivates ribosomes by degrading an adenine residue at position 4324 in 28S rRNA. Recently, we have demonstrated that pterin-7-carboxamides with peptide pendants were potent RTA inhibitors. Among these, -(pterin-7-carbonyl)glycyl-L-tyrosine () is the most potent RTA inhibitor as a small organic molecule. However, despite this fascinating inhibitory activity, the mode of interaction of with RTA remains elusive. This study aimed to elucidate the factors responsible for the high RTA inhibitory activity of based on X-ray crystallographic analysis. Herein, we report the successfully resolved X-ray crystal structure of /RTA complexes, revealing that the interaction between the phenolic hydroxy group in and Asn78 of RTA through a hydrogen bonding and the conformational change of Tyr80 and Asn122 are responsible for the high RTA inhibitory activity of .

PubMed: 37259593
DOI: 10.1080/14756366.2023.2219038

Experimental method

X-RAY DIFFRACTION (1.6 Å)