8I54

Lb2Cas12a RNA DNA complex

8I54 の概要

• エントリーDOI: 10.2210/pdb8i54/pdb
• EMDBエントリー: 35191
• 分子名称: Lb2Cas12a, RNA (33-MER), DNA (25-MER), ... (4 entities in total)
• 機能のキーワード: complex, antimicrobial protein
• 由来する生物種: Lachnospiraceae bacterium MA2020; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 162178.66

構造登録者

Li, J.,Sivaraman, J.,Satoru, M. (登録日: 2023-01-24, 公開日: 2023-02-22, 最終更新日: 2024-07-03)

主引用文献

Jianwei, L.,Jobichen, C.,Machida, S.,Meng, S.,Read, R.J.,Hongying, C.,Jian, S.,Yuan, Y.A.,Sivaraman, J.
Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.
Plos Biol., 21:e3002023-e3002023, 2023

PubMed Abstract: Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR-Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well as the cryo-EM structure and functional studies of the Lb2Cas12a+crRNA+DNA complex. We demonstrate that apo Lb2Cas12a assumes a unique, elongated conformation, whereas the Lb2Cas12a+crRNA binary complex exhibits a compact conformation that subsequently rearranges to a semi-open conformation in the Lb2Cas12a+crRNA+DNA ternary complex. Notably, in solution, apo Lb2Cas12a is dynamic and can exist in both elongated and compact forms. Residues from Met493 to Leu523 of the WED domain undergo major conformational changes to facilitate the required structural rearrangements. The REC lobe of Lb2Cas12a rotates 103° concomitant with rearrangement of the hinge region close to the WED and RuvC II domains to position the RNA-DNA duplex near the catalytic site. Our findings provide insight into crRNA recognition and the mechanism of target DNA cleavage.

PubMed: 36917574
DOI: 10.1371/journal.pbio.3002023

実験手法

ELECTRON MICROSCOPY (3.95 Å)