8I4Q

Crystal structure of 6-phosphogluconate dehydrogenase from Corynebacterium glutamicum

Summary for 8I4Q

• Entry DOI: 10.2210/pdb8i4q/pdb
• Descriptor: 6-phosphogluconate dehydrogenase, decarboxylating, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (5 entities in total)
• Functional Keywords: nadp dependent enzyme, oxidoreductase
• Biological source: Corynebacterium glutamicum ATCC 13032
• Total number of polymer chains: 2
• Total formula weight: 107977.60

Authors

Yu, H.,Kim, K.-J. (deposition date: 2023-01-20, release date: 2023-07-26, Last modification date: 2024-05-29)

Primary citation

Yu, H.,Hong, J.,Seok, J.,Seu, Y.B.,Kim, I.K.,Kim, K.J.
Crystal Structures of 6-Phosphogluconate Dehydrogenase from Corynebacterium glutamicum.
J Microbiol Biotechnol., 33:1-9, 2023

PubMed Abstract: () has been considered a very important and meaningful industrial microorganism for the production of amino acids worldwide. To produce amino acids, cells require nicotinamide adenine dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from ATCC 13032 (6PGD) and reported our biological research based on this structure. We identified the substrate binding site and co-factor binding site of 6PGD, which are crucial for understanding this enzyme. Based on the findings of our research, 6PGD is expected to be used as a NADPH resource in the food industry and as a drug target in the pharmaceutical industry.

PubMed: 37417004
DOI: 10.4014/jmb.2305.05002

Experimental method

X-RAY DIFFRACTION (1.9 Å)