8HT7
The N-terminal region of Cdc6 specifically recognizes human DNA G-quadruplex
Summary for 8HT7
Entry DOI | 10.2210/pdb8ht7/pdb |
Descriptor | DNA (5'-D(*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*TP*GP*GP*G)-3'), GLN-ALA-GLN-ALA-THR-ILE-SER-PHE-PRO-LYS-ARG-LYS-LEU-SER-TRP (2 entities in total) |
Functional Keywords | dna replication, g-quadruplex, intrinsically disordered region, dna binding protein |
Biological source | Homo sapiens More |
Total number of polymer chains | 2 |
Total formula weight | 8425.33 |
Authors | |
Primary citation | Geng, Y.,Liu, C.,Xu, N.,Shi, X.,Suen, M.C.,Zhou, B.,Yan, B.,Wu, C.,Li, H.,Song, Y.,Chen, X.,Wang, Z.,Cai, Q.,Zhu, G. The N-terminal region of Cdc6 specifically recognizes human DNA G-quadruplex. Int.J.Biol.Macromol., 260:129487-129487, 2024 Cited by PubMed Abstract: Guanine (G)-rich nucleic acid sequences can form diverse G-quadruplex structures located in functionally significant genome regions, exerting regulatory control over essential biological processes, including DNA replication in vivo. During the initiation of DNA replication, Cdc6 is recruited by the origin recognition complex (ORC) to target specific chromosomal DNA sequences. This study reveals that human Cdc6 interacts with G-quadruplex structure through a distinct region within the N-terminal intrinsically disordered region (IDR), encompassing residues 7-20. The binding region assumes a hook-type conformation, as elucidated by the NMR solution structure in complex with htel21T. Significantly, mutagenesis and in vivo investigations confirm the highly specific nature of Cdc6's recognition of G-quadruplex. This research enhances our understanding of the fundamental mechanism governing the interaction between G-quadruplex and the N-terminal IDR region of Cdc6, shedding light on the intricate regulation of DNA replication processes. PubMed: 38237821DOI: 10.1016/j.ijbiomac.2024.129487 PDB entries with the same primary citation |
Experimental method | SOLUTION NMR |
Structure validation
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