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8HT7

The N-terminal region of Cdc6 specifically recognizes human DNA G-quadruplex

Summary for 8HT7
Entry DOI10.2210/pdb8ht7/pdb
DescriptorDNA (5'-D(*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*TP*GP*GP*G)-3'), GLN-ALA-GLN-ALA-THR-ILE-SER-PHE-PRO-LYS-ARG-LYS-LEU-SER-TRP (2 entities in total)
Functional Keywordsdna replication, g-quadruplex, intrinsically disordered region, dna binding protein
Biological sourceHomo sapiens
More
Total number of polymer chains2
Total formula weight8425.33
Authors
Liu, C.,Zhu, G.,Geng, Y.,Xu, N. (deposition date: 2022-12-20, release date: 2023-12-27, Last modification date: 2024-05-22)
Primary citationGeng, Y.,Liu, C.,Xu, N.,Shi, X.,Suen, M.C.,Zhou, B.,Yan, B.,Wu, C.,Li, H.,Song, Y.,Chen, X.,Wang, Z.,Cai, Q.,Zhu, G.
The N-terminal region of Cdc6 specifically recognizes human DNA G-quadruplex.
Int.J.Biol.Macromol., 260:129487-129487, 2024
Cited by
PubMed Abstract: Guanine (G)-rich nucleic acid sequences can form diverse G-quadruplex structures located in functionally significant genome regions, exerting regulatory control over essential biological processes, including DNA replication in vivo. During the initiation of DNA replication, Cdc6 is recruited by the origin recognition complex (ORC) to target specific chromosomal DNA sequences. This study reveals that human Cdc6 interacts with G-quadruplex structure through a distinct region within the N-terminal intrinsically disordered region (IDR), encompassing residues 7-20. The binding region assumes a hook-type conformation, as elucidated by the NMR solution structure in complex with htel21T. Significantly, mutagenesis and in vivo investigations confirm the highly specific nature of Cdc6's recognition of G-quadruplex. This research enhances our understanding of the fundamental mechanism governing the interaction between G-quadruplex and the N-terminal IDR region of Cdc6, shedding light on the intricate regulation of DNA replication processes.
PubMed: 38237821
DOI: 10.1016/j.ijbiomac.2024.129487
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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