8HP8

Crystal Structure of Engineered Endolysin EC340 derived from Gram-Negative Bacteria targeted Bacteriophage

Summary for 8HP8

Entry DOI	10.2210/pdb8hp8/pdb
Descriptor	Endolysin mtEC340M (2 entities in total)
Functional Keywords	endolysin, gram-negative bacteria, bacteriophage, viral protein, hydrolase
Biological source	Escherichia phage PBEC131
Total number of polymer chains	3
Total formula weight	51016.19
Authors	Wang, J.M.,Yoon, W.S.,Seo, M.D. (deposition date: 2022-12-12, release date: 2023-05-17, Last modification date: 2024-05-29)
Primary citation	Wang, J.M.,Seok, S.H.,Yoon, W.S.,Kim, J.H.,Seo, M.D. Crystal structure of the engineered endolysin mtEC340M. Acta Crystallogr.,Sect.F, 79:105-110, 2023 Cited by PubMed Abstract: Endolysins produced by bacteriophages play essential roles in the release of phage progeny by degrading the peptidoglycan layers of the bacterial cell wall. Bacteriophage-encoded endolysins have emerged as a new class of antibacterial agents to combat surging antibiotic resistance. The crystal structure of mtEC340M, an engineered endolysin EC340 from the PBEC131 phage that infects Escherichia coli, was determined. The crystal structure of mtEC340M at 2.4 Å resolution consists of eight α-helices and two loops. The three active residues of mtEC340M were predicted by structural comparison with peptidoglycan-degrading lysozyme. PubMed: 37132476 DOI: 10.1107/S2053230X23002583 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.397 Å)

Structure validation