8HJ4
CryoEM structure of an anti-CRISPR protein AcrIIC5 bound to Nme1Cas9-sgRNA complex
Summary for 8HJ4
Entry DOI | 10.2210/pdb8hj4/pdb |
EMDB information | 34832 |
Descriptor | CRISPR-associated endonuclease Cas9, sgRNA, Phage protein (3 entities in total) |
Functional Keywords | cas9, anti-crispr proteins, cleavage inhibition, antimicrobial protein, hydrolase-rna-antimicrobial protein complex, hydrolase/rna/antimicrobial protein |
Biological source | Neisseria meningitidis serogroup C (strain 8013) More |
Total number of polymer chains | 3 |
Total formula weight | 183160.15 |
Authors | |
Primary citation | Sun, W.,Zhao, X.,Wang, J.,Yang, X.,Cheng, Z.,Liu, S.,Wang, J.,Sheng, G.,Wang, Y. Anti-CRISPR AcrIIC5 is a dsDNA mimic that inhibits type II-C Cas9 effectors by blocking PAM recognition. Nucleic Acids Res., 51:1984-1995, 2023 Cited by PubMed: 36744495DOI: 10.1093/nar/gkad052 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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